Introduction Plants and animals both have a layer of tissue dubbed the epidermal layer. Plants have special pores called stomata to enable passage that material. The stomata pores room surrounded ~ above both political parties by jellybean shame cells referred to as guard cells. Unlike various other plant epidermal cells, the guard cell contain chlorophyll to execute photosynthesis. This allows the cell to expand/ contract to open up or nearby the stomata. Safety cells additionally close once dehydrated. This keeps water in the plant from escaping. The opening or closing of guard cells deserve to be viewed in a microscope by including different water concentration to the sheet tissue.

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most stomata are on the lower epidermis the the leaves on plants (bottom the the leaf). The number of stomata top top the epidermal surface deserve to tell you a lot about a plant. Usually, a high concentration the stomata indicates quick growth and wet climate. Lower concentrations of stomata suggest lower prices of photosynthesis and also growth or adaptations because that dry weather.

Purpose: to view and also compare the stomata indigenous the leaves of several types of plant

Materials: 3 leaves (1 from 3 various species), compound light microscope, 3 microscopic lense slides, clear pond polish, transparent tape


Obtain three leaves from different species of plants.Paint a special patch (at least one square centimeter) the clear pond polish ~ above the underside that the leaf surface ar being studied.Allow the pond polish to dried completely.Tape a piece of clear cellophane tape come the dried nail polish patch.Gently peel the pond polish patch from the sheet by pulling ~ above a edge of the tape and “peeling” the fingernail polish off the leaf. This is the sheet impression you will certainly examine.Tape her peeled impression to a really clean microscopic lense slide. Use scissors come trim away any type of excess tape. Brand the slide through plant name.Examine the leaf impression under a light microscopic lense at 400X.Search for areas where there are plenty of stomata, and also where there space no dirt, ignorance prints, damaged areas, or big leaf veins. Attract the leaf surface ar with stomata. Count all the stomata in one microscope field. Record the number on her data table.Repeat counts for at least three other unique microscopic fields. Document all the counts. Identify an typical number per microscopic field.From the average number/400X microscope field, calculation the stomata every mm2 by multiplying by 8.Follow procedures 2 – 11 through the various other leaves.

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 Leaf 1 Leaf 2 Leaf 3
Name of Leaf
Drawing in 400x (with numerous stomata) 

Stomata in ar 1 
Stomata in field 2 
Stomata in ar 3 
Average Stomata in field 
Stomata/ mm2 


1. I beg your pardon leaf had actually the most stomata? Why carry out you think this was so?

2. Explain, in detail, exactly how guard cell open and also close stomata?

3. At what time the day would stomata it is in closed and also why?

4. Why go the lower epidermis have much more stomata than the upper epidermis of a leaf?

5. Specify transpiration.

6. What two gases relocate in and out the the sheet stomata?

7. What go a larger variety of leaf stomata indicate around the growing climate of that plant?

8. Would you expect cam plants to have as many stomata? Why or why not?


Author biology Junction TeamPosted ~ above April 21, 2017April 15, 2019Categories Uncategorized

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