e. Acid-fast stain for microbes v waxy cell walls*This stain is very useful in identify Mycobacterium spp. The exhibit waxy cell walls.
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Consider the adhering to situations, and determine once a straightforward stain can be used rather of a Gram stain.a. A microbiologist is trying to identify whether a specimen includes bacteria or fungi.b. An unknown types of bacteria is gift characterized and also identified.c. A specimen is doubt to contain multiple varieties of bacteria.d. A wound specimen is gift examined.
a. A microbiologist is trying to identify whether a specimen includes bacteria or fungi.*A an easy stain is provided to visualize the entire organism and also highlight cellular shapes or structures. In this case, differentiation the bacterial features is no necessary.
A student creates a Gram stain on a bacter sample that has a mix the gram-negative and also gram-positive organisms. The student accidentally forgets the decolorizer step. What would certainly the result of the Gram stain be?a. The Gram stain would show pink (gram-negative bacteria) and purple (gram-positive bacteria).b. Every organisms would appear colorless.c. All organisms would show up pink (gram-negative).d. Every organisms would appear purple (gram-positive).
d. Every organisms would show up purple (gram-positive).*Without the decolorizer step, all the organisms maintain the violet stain.
What is the function of iodine in the Gram stain process?a. Iodine is the decolorizer remove the main stain after ~ the first step in the Gram stain technique.b. Iodine is a mordant in the Gram stain technique, which functions to increase the main stain.c. Iodine is a pink dye that acts together the major stain in the Gram stain technique.d. Iodine is a simple purple dye, i m sorry acts together the main stain in the Gram stain technique.
In microscopy, the term resolutiona. Refers to magnification once using the electron microscope.b. Is the very same as the complete magnification that a specimen.c. Is just observed in stained specimens.d. Describes the capability to distinguish fine structure and detail in a specimen.e. Is boosted when much longer wavelengths that light room employed.
Bacterial smears room fixed prior to staining toa. Do the cell visible.b. Expropriate stain.c. Affix the cell to the slide.d. Make their walls permeable.
You find colorless areas in cells in a Gram-stained smear. What should you use next?a. A capsule stainb. A flagella stainc. An acid-fast staind. An endospore staine. A basic stain
Assume you stain Bacillus by applying malachite green with heat and then counterstaining through safranin. With the microscope, the green structures area. Flagella.b. Capsules.c. Cell walls.d. Endospores.e. The answer can not be determined.
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Which kind of stain is most advantageous in help clinicians to decide which antibiotic to prescribe because that a bacter infection?a. Negative stainb. Straightforward stainc. Gram staind. Flagella staine. Endospore stain
You room performing a Gram stain ~ above gram-positive bacteria and you stop after the addition of the counterstain. What is the figure of the bacteria in ~ this point?a. Purpleb. Redc. Colorlessd. Brown
You space performing a Gram stain on gram-negative bacteria and also you avoid after the decolorizer step. What is the appearance of the bacteria in ~ this point?a. Purpleb. Redc. Colorlessd. Brown
You are performing a Gram stain top top gram-negative bacteria and you prevent after the addition of the mordant. What is the illustration of the bacteria in ~ this point?a. Purpleb. Redc. Colorlessd. Brown
Which step in the Gram stain is the vital step in distinguishing gram-positive cells from gram-negative cells?a. Iodineb. Decision violetc. Alcohol-acetoned. Safranin
You room performing a Gram stain on gram-positive bacteria and also you protect against after the addition of the first dye. What is the figure of the bacteria in ~ this point?a. Purpleb. Redc. Colorlessd. Brown
Simple staining is often crucial to improve contrast in i beg your pardon microscope?a. Fluorescence microscopeb. Link light microscopec. Electron microscoped. Darkfield microscopee. Phase-contrast microscope
The negative stain is supplied toa. Recognize Gram reaction.b. Recognize flagella arrangement.c. Visualize the external membrane.d. Visualize endospores.e. Identify cell size.